Some people use TE buffers with different pH's for different applications.Some protocols use TE 10:0.1 with 0.1 mM EDTA to reduce the interaction of the EDTA with downstream applications.Each EDTA molecule chelates one Mg 2+ ion. So, when using DNA or RNA that was suspended in TE, you should keep track of the amount of EDTA in the mix to make sure there is still enough Mg 2+ for subsequent reactions to proceed successfully. However, downstream reactions like restriction digests, PCR, ligations, and reverse transcription typically require Mg 2+, potentially making the presence of EDTA in the reaction problematic. The EDTA in TE chelates Mg 2+ and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes. TE buffer is often used to store DNA and RNA.For the Tris-HCl use Tris base and adjust to desired pH using HCl.→ 1x TE is 10 mM Tris-HCl and 1 mM EDTA Notes Recipe 1x TE for 1 liter of 1x TE solution volume Recipe 10x TE for 1 liter of 10x TE stock solution volume Tris is a buffering agent to keep the solution at a defined pH.EDTA in TE chelates Mg 2+ and other divalent metals ions necessary for most causes of DNA and RNA degradation, suppressing these processes.
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